Order #29-005030, #29-005040
This protocol is used for double staining with UranyLess and lead citrate on ultrathin sections.
The protocol is adapted for biological samples that have been fixed with glutaraldehyde, osmium or ruthenium and embedded in an epoxy type resin (i.e. Epon, Araldite, Spurr) or an acrylic type resin (LRWhite, HM20).
Staining protocol:
Place a droplet of Stain section Blot with filter paper Rinse in distilled water
UranyLess on parafilm 1-2 minutes and let sample dry
After drying, use the lead citrate protocol according to Reynolds (1963).
Technical tips:
This protocol is adapted for negative staining with UranyLess. It allows characterisation of isolated particles or morphology of bacteria, viruses, protein, liposomes, exomes, nanoparticles etc.
Staining protocol:
Place a sample droplet | Place grid onsample droplet | Blot with filter paper | Place the grid on UranyLess | Blot with filterpaper |
Technical Tip:
TSB 28-005030 UranyLess classic and negative staining protocols, Revision 1